Uberstrainer

Filtration of large sample volumes is easy with a Re-Strainer and Pluristrainer. Continue reading to learn more about how both of them work. 

It is essential to correctly prepare the sample in order to achieve a smooth cell flow. The type of cells to be examined, as well as any other potential impediments to the flow, must be considered.

Cell strainers are sterile, user-friendly devices used to obtain uniform cell suspension from tissues. It is used as a more convenient and faster alternative to gauze filtration. They are ideal for preparing flow cytometry samples and filtering agglutinative proteins generated during serum inactivation.

Our best Lab Cell Strainers are made of a strong nylon mesh with evenly spaced mesh pores. They are sterile, simple devices for quickly isolating primary cells and obtaining a uniform single-cell suspension from tissues. Designed to protect expensive flow cytometry and cell sorting equipment by removing clumps and debris from cell suspensions and clinical samples prior to analysis.

How Do The Cell Strainers Work?

Cell strainers separate debris or clumps from samples, allowing cells to be isolated for further examination. To create a tight fit, the cell strainers fit on the tops of various size sample tubes, so the material must pass through the strainer.

Only certain materials are permitted to pass through the mesh, which is made up of varying sizes. It enables material separation based on size, allowing for the isolation of specific material from the rest of the sample.

What is a Re-Strainer?

The Re-Strainer is a high-volume liquid filtration device that can exclude or concentrate particles from liquids while also allowing for particle recovery. Unscrew the cap to gain access to the 6 ml recovery reservoir. There are two female Luer-lock ports on the Re-Strainer.

These ports can be used to connect multiple Re-Strainers to a filter cascade (male to male Luer-lock connector) or to incorporate the Re-Strainer as an in-line-strainer using a Luer-lock tube adaptor.

The mesh is an integral part of the housing, providing maximum mechanical stability to the device.

Applications of Re-strainer

• Large sample volume filtration
• Filtration in a cascade
• Particle size fractionation
• Smaller impurities are removed.
• Rare particle concentrations from large volumes
• Affinity chromatography gel cartridge

Other specifications

• Flow Control: yes
• Mesh Size: 1 μm
• Mesh Fixation: Injected in housing for stronghold
• Fabric material: PET (Polyethylenterephthalat)
• Housing Material: LD-PE (Low-Density Polyethylene)
• Sterility: Sterile
• Stability: Good resistance to non-oxidizing acids and bases, fats, and most organic solvents
• Storage Condition: Room Temperature

What is a PluriStrainer?

The pluriStrainer is a sieving device (or cell strainer) used in a variety of laboratory applications that require liquid filtration and purification. Its one-of-a-kind design includes improved ventilation to prevent clogging for smart sample preparation. PluriStrainer can be stacked to allow direct filtration with various mesh sizes and can also be inverted to recover the sieved material. 

It can be combined with a funnel for larger sample volumes. It is compatible with all major brand 50 mL centrifuge (conical) tubes. The combination of the pluriStrainer and the Connector Ring enables the use of low pressure, which aids in filtration. 

Furthermore, the liquid on top of the mesh can be held back by blocking the flow through the sieve. The liquid on top of the mesh can be used for sample preparation and incubation, such as tissue dissociation or cell lysis (e.g., Buffer RLT, TRIzol).

When should you use Pluristrainer?

• The actual single-cell suspension is produced following the enzymatic digestion of mammary tissue and organoids.
• Physical dissociation of the primary tissue
• Single-cell suspensions of blood cells from the thymus, pancreas, lymph nodes, bone marrow, and other sources are prepared.
• Preparation of a real single-cell suspension for flow cytometry 
• Using gauze for filtering is a quick and simple solution.
• Incubation of cells/cytokines for a short period of time (in combination with pluriBead and Connector Ring)
• Complex and viscous liquids, such as fat tissue, are sieved (in combination with a low-pressure syringe and a Connector Ring).

What are the applications of PluriStrainer?

PluriStrainer

Attach the 50 mL tube to the pluriStrainer.

On top of that, place your sample material. Your sample material will only be processed in part. By inserting a syringe into the Connector Ring, you can force low pressure to support the straining of rough sample material while pulling the piston.

Stacked pluriStrainer

PluriStrainers are available in a variety of mesh sizes that can be stacked for cascade straining of multiple cell sizes at the same time. 

Reversed pluriStrainer

To obtain the larger fraction, remove the pluriStrainer, turn it upside down on another 50 ml tube, and flush the sample back into the pluriStrainer. 

PluriStrainer with Connector Ring 

It lets you control the flow rate by opening and closing the Luer-Lock, which is useful for physical tissue dissociation (brain, spleen, etc.). 

The sample material will flow through when you open the Luer-Lock. 

PluriStrainer with Funnel

Insert a funnel into the pluriStrainer (42-50000-03). 

You can also add up to 24 mL of sample material.

Connector Ring, PluriStrainer, and Syringe

By attaching a syringe to the Connector Ring, you can apply low pressure to aid in the straining of rough sample material while pulling the piston.

Specifications

• Mesh Size: 1 – 500 μm
• Mesh Material: PET
• Mesh Type: Woven
• Housing Material: LD-PE (Low-Density Polyethylen)
• Sterility: Gamma Ray Sterilized

Key features

• Sieving is a straightforward process with mesh sizes ranging from 1 to 500 m.
• Reverse the procedure to obtain the larger fraction. 
• Targets of various sizes may be stacked. 
• A funnel is needed for larger sample volumes.
• Connector Ring for Flow Control
• Low pressure is used to aid sieving.

Are you looking for the best lab cell strainer for your research? Visit our website: www.uberstrainer.com to see which high-quality products you require for cell or particle separation. Take a look at the Programs & Special Offers section as well.

cytotoxic, helper, and regulatory T cells are the three types of T cells. Learn more about our unique Effector T cell separation technologies.

Effector cells are immune system cells that have gone through the process of differentiation and maturation. In the event of a stimulus, these are the cells that mount specific responses. 

As part of the immune response against a pathogen or a self cell, the body’s immune system generates effector cells (in case of autoimmune disorders).

We’ll look at how Pluribead cell separation aids in Effector T Cells isolation.

T Cell Activation

T cell activation is a prerequisite for the majority of immune responses, and normally, immune reactions to foreign antigens are carefully regulated by communication between antigen-specific T cells and antigen-presenting cells. Naive T cells need to be activated by a number of signals. 

One signal is antigen-specific and is given off when the TCR is active. Co-stimulatory molecules and their interactions produce additional signals. As a result of their interactions with T cells, resting antigen-presenting cells, such as resting B cells, frequently do not express high levels of co-stimulatory molecules. 

The interactions of CD28 with B7-1 (CD80) and B7-2 (CD86) and CD40 ligands with CD40 are two of the most significant co-stimulatory systems. These two interconnected systems of molecules also affect each other.

Effector Cells

Effector cells are a special kind of cell that perform a particular activity when stimulated in the body. The term “effector cell” is typically used to describe specific immune system cells, but it is also occasionally used to describe nervous system cells that are located at the ends of autonomic nerve terminals and that, when activated, perform a specific function. 

Effector cells in the immune system are the activated, relatively transient cells that protect the body during an immune response. Activated T cells include cytotoxic T cells and helper T cells, which carry out cell-mediated reactions, and effector B cells, also known as plasma cells, which secrete antibodies.

Cytotoxic T Cells

The primary function of cytotoxic T cells, also known as CD8+ cells, is to kill toxic/target cells. When they are recognized, their purpose changes to the removal of virally infected cells, bacteria, and tumor fragments (such as cancer cells) via a process known as apoptosis. Apoptosis occurs when the internal organelles of a cell are destroyed, causing the cell to die from the inside out.

Helper T Cells

T helper cells, also known as CD4+ cells, are similar to cytotoxic cells but perform a broader range of functions. These cells are critical to cell immunity because they are required for the majority of adaptive immune responses. When exposed to antigens, T helper cells become activated and have the ability to differentiate into cell subtypes. 

However, the ability of helper T cells to function depends on their activation to become effector cells. The innate immune responses brought on by an infection activate them on the surface of antigen-presenting cells. 

The innate responses also control the type of effector cell that a helper T cell will mature into, which in turn controls the type of adaptive immune response that is elicited.

Regulatory T Cells

The regulatory T cell is the final type of effector cell. Once the threat has been eliminated, regulatory T cells are tasked with suppressing the autoimmune response. 

After helper T cells and cytotoxic T cells bind to a pathogen and work together to eliminate it from the body, they are no longer useful. Regulatory T cells prevent them from taking up space or attacking healthy cells until they die of apoptosis.

While these three types of effector cells handle the majority of the immune response, they are not the only T cell variations. Even after a pathogen has been removed, some types of T lymphocytes remain. 

These long-living lymphocytes are memory T cells that are highly capable of responding to antigens when reintroduced—which aids the immune system.

These cells are formed following infection and are extremely important because they have the ability to multiply into a large number of effector T cells when exposed to familiar antigens. Memory cells are distinct in that they remember pathogens and infectious cells faster than other cells, allowing them to fight bacteria and viruses more effectively.

Try Our Cell Separation Products Right Now!

Pluribead, a leading cell enrichment technology, is specifically designed for fast and gentle sample preparation, resulting in a highly-enriched population of healthy, viable cells. 

Two Different Bead Sizes are Available

• S-pluriBead: It is applied to a limited number of targets in large sample volumes.
• M-pluriBead: A material that is versatile and can be used to achieve multiple goals while using less material.

Key features of Pluribead

• No Sample Preparation: Utilize a sample volume of 200 l to 45 ml without the use of erythrolysis, gradient centrifugation, or any other techniques.
• Use any type of sample material, including PBMC, secretion/excretion material, brain homogenate, spleen, liver, buffy coat, whole blood, and so on. 
• Applicable to a Wide Variety of Species: Isolate from sheep, mice, rats, cows, dogs, and other animals. 
• Quick Isolation: Cell division can begin in five minutes.
• Simultaneous Cell Isolation Using PluriBead Cascade: Separate two different cell types simultaneously from the same sample material.
• Sequential Cell separation: Utilizing sequential cell isolation, isolate up to six different targets from a single sample.

Learn more about our T cell separation products, which can help you overcome long-standing sample preparation challenges.